The Effects of a Training Session on Teacher Knowledge, Perceptions, and Implementation of Assistive Technology in Secondary Schools.

Despite the prevalence of students with mild disabilities in special education and the legal mandate to consider assistive technology to support their needs, research suggests low rates of assistive technology use by this population (Bouck, Maeda, & Flanagan, 2012; Derer, Polsgrove, & Rieth, 1996; Quinn, Behrmann, Mastropieri, & Chung, 2009). One major barrier to assistive technology consideration and implementation cited by teachers is a lack of training. This study examined changes in teachers’ knowledge, perceptions, and implementation of assistive technology as a result of a hands-on teacher training session. Participants included 61 regular and special education teachers and administrators in secondary public schools in one Western state. Participants completed a pre-training survey measuring their knowledge and experience with assistive technology, as well as their attitudes about using assistive technology. They then participated in a hands-on training session about assistive technology held at their school. After the training session, participants completed a post-training survey, as well as a follow-up survey given 30 days after the training session. The follow-up survey measured changes in participant implementation of assistive technology following the training session. Results of this study show that a teacher training session improved regular and special education teachers’ and administrators’ knowledge and perceptions of assistive technology. The findings also show that 49% of respondents to the follow up survey reported using assistive technology in their classrooms following the training session. Implications for future research are discussed

DNA Methyltransferase Controls Stem Cell Aging by Regulating BMI1 and EZH2 through MicroRNAs

Epigenetic regulation of gene expression is well known mechanism that regulates cellular senescence of cancer cells. Here we show that inhibition of DNA methyltransferases (DNMTs) with 5-azacytidine (5-AzaC) or with specific small interfering RNA (siRNA) against DNMT1 and 3b induced the cellular senescence of human umbilical cord blood-derived multipotent stem cells (hUCB-MSCs) and increased p16INK4A and p21CIP1/WAF1 expression. DNMT inhibition changed histone marks into the active forms and decreased the methylation of CpG islands in the p16INK4A and p21CIP1/WAF1 promoter regions. Enrichment of EZH2, the key factor that methylates histone H3 lysine 9 and 27 residues, was decreased on the p16INK4A and p21CIP1/WAF1 promoter regions. We found that DNMT inhibition decreased expression levels of Polycomb-group (PcG) proteins and increased expression of microRNAs (miRNAs), which target PcG proteins. Decreased CpG island methylation and increased levels of active histone marks at genomic regions encoding miRNAs were observed after 5-AzaC treatment. Taken together, DNMTs have a critical role in regulating the cellular senescence of hUCB-MSCs through controlling not only the DNA methylation status but also active/inactive histone marks at genomic regions of PcG-targeting miRNAs and p16INK4A and p21CIP1/WAF1 promoter regions

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Effects of digoxin on muscle reflexes in normal humans.

Blockade of the skeletal muscle Na(+)-K(+)-ATPase pump by digoxin could result in a more marked hyperkaliema during a forearm exercise, which in turn could stimulate the mechano- and metaboreceptors. In a randomized, double-blinded, placebo-controlled, and cross-over-design study, we measured mean blood pressure (MBP), heart rate (HR), ventilation (V(E)), oxygen saturation (SpO(2)), muscle sympathetic nerve activity (MSNA), venous plasma potassium and lactic acid during dynamic handgrip exercises, and local circulatory arrest in 11 healthy subjects. Digoxin enhanced MBP during exercise but not during the post-handgrip ischemia and had no effect on HR, V(E), SpO(2), and MSNA. Venous plasma potassium and lactic acid were also not affected by digoxin-induced skeletal muscle Na(+)-K(+)-ATPase blockade. We conclude that digoxin increased MBP during dynamic exercise in healthy humans, independently of changes in potassium and lactic acid. A modest direct sensitization of the muscle mechanoreceptors is unlikely and other mechanisms, independent of muscle reflexes and related to the inotropic effects of digoxin, might be implicated.Journal ArticleRandomized Controlled TrialResearch Support, Non-U.S. Gov'tinfo:eu-repo/semantics/publishe